The application of TOOS in in in vitro diagnostic reagents

TOOS (CAS No. 82692-93-1), Chinese name: N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, is a white or slightly blue crystalline powder. It is an important member of the new Trinder's reagent family and a novel highly water-soluble aniline derivative widely used in diagnostic and biochemical tests. 1. Blood glucose testing items: TOOS is used in the blood glucose metabolism project to prepare glucose testing reagents, glycated albumin testing reagents, etc. According to patent CN105572065A, glucose oxidase is used to catalyze the oxidation of glucose to generate hydrogen peroxide. Platinum bismuth nano mimetic peroxidase catalyzes the oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS) to produce color. The color of the color product solution is purple, and as the glucose concentration increases, the color of the solution deepens, The maximum absorption wavelength of the colorimetric system is 590nm, and the glucose content can be determined by the absorbance at 590nm. 2. Routine liver function examination items: Adenosine deaminase (ADA) activity is a sensitive indicator reflecting liver injury and is one of the routine liver function examination items. TOOS, bovine serum albumin, and disodium ethylenediaminetetraacetate constitute adenosine deaminase detection reagents, which have the advantages of good colorimetric effect, rapid response, stability, and high measurement precision.